Fig 1: H2O2 induces BAG3 reduction.A Immunoblot results indicated H2O2 treatment caused time and dosage-dependent decreases in neuronal BAG3. B qPCR demonstrated H2O2 treatment causes significant decreases in neuronal BAG3 RNA. C In addition to validating H2O2-induced reductions in BAG3 protein, immunocytochemistry suggested neurons exposed to H2O2 treatment may develop minor dystrophies in their branching processes. D Immunoblotting revealed H2O2 reduced other BAG family members (BAG2, BAG5, BAG6), and exacerbated the reduction in BAG3 observed in response to Tat or Nef. ***p < 0.001. Scale bar equals 50 µM; ×40 magnification. Scale bar equals 20 µM; ×63 magnification.